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Taxonomic Considerations. The recent discovery that Brachycephalus is allied with the eleutherodactylines (1, 2) has made uncertain the valid family name for Eleutherodactylus and its relatives. However, the decision to place all ~800 species of eleutherodactylines in the family Brachycephalidae (2, 3) was based on analysis of 16 eleutherodactyline species, only six of which were from South America (2). In our analyses here of many more species, Brachycephalus appears in a basal position in the trees and does not group significantly with any species or clade of eleutherodactylines (SI Figs. 5-13). The association of Brachycephalus with the eleutherodactyline lineage, in itself, does not require the use of the family name Brachycephalidae for the entire assemblage. If multiple families are recognized, the correct allocation of these names will depend on a more robust phylogeny, especially concerning the basal branches. For example, Brachycephalidae might be appropriate only for Brachycephalus, with one or more other family names applied to the eleutherodactylines. Also, the suggestion by Frost et al. (2) that two genera of eleutherodactylines might be close relatives of Brachycephalus was not supported in a recent morphological study (4). For these reasons, we use Brachycephalidae as a family name only for Brachycephalus and refer in this current study to the remaining assemblage of genera and species as "eleutherodactyline" frogs, pending further study.

Assignment of unsampled species to the major clades (genera) defined here (SI Table 2) was based largely on their species-group affiliation. Although our analyses show many changes in content for previously defined species groups, most species assigned to the same species group are placed here in the same genus. Exceptions include Phrynopus and species from southeast Brazil, which will require further systematic study (see below).

We restrict the generic name Eleutherodactylus Duméril and Bibron, 1841 (type species, Hylodes martinicensis) to the Caribbean Clade and continue recognition of the included subgenera. The subgenus Eleutherodactylus is now restricted to the West Indies, and Syrrhophus includes two species in Cuba (E. symingtoni and E. zeus), besides those in Middle America. Eleutherodactylus counouspeus will be assigned to a new subgenus elsewhere. We consider Eleutherodactylus planirostris rogersi as a species (Eleutherodactylus rogersi) distinct from E. planirostris because they were not found to be closest relatives.

Because sequence data are unavailable for the type species of Phrynopus Peters, 1873 (Phrynopus peruanus), it is not possible to allocate that generic name to the separate clades defined here as including species of "Phrynopus," and, therefore, we make no changes in their generic status. The molecular phylogeny (Fig. 2) places into question the validity of the morphological species groups, restricting us from making any assumptions about species believed to be closely related to P. peruanus based on morphology.

The Southeast Brazil Clade is a similar situation in that we lack sequence data for the type species (Eleutherodactylus verrucosus) of the earliest possible generic name for the group, Ischnocnema Reinhardt and Lütken, 1862. However, in this case, we have confidence that the generic name Eleutherodactylus (The Caribbean Clade) does not apply to any of the species, based on geography and morphology (5-7). Also, our analyses (Fig. 2) indicate that E. binotatus and, by implication, its close relative E. plicifer, belong to a separate genus that we will name elsewhere. These two species also have an unusually long first finger which appears to set them apart from almost all of the other species of Eleutherodactylus in southeast Brazil except E. paulodutrai and E. ramagii, which differ significantly in other characters (5, 6). Although the number of species in this study from the Southeast Brazil Clade is small (four), they represent several clusters of species, each sharing a suite of morphological traits indicating relatedness (5-9). By implication, this increases the size of the clade to a minimum of 12 species: E. epipedus, E. erythromerus, E. gualteri, E. guentheri, E. hoehnei, E. izecksohni, E. juipoca, E. nasutus, E. oeus, E. parvus, E. pusillus, and E. vinhai.

Until additional data are collected, we cannot say with confidence that E. verrucosus and the remaining species of Eleutherodactylus in southeastern Brazil belong to this Southeast Brazil Clade. Nonetheless, those remaining species form several clusters themselves (e.g., lacteus group and ramagii group) and some species, in the past, have been placed in the same morphological species group (6) along with species that we confidently place in the Southeast Brazil Clade. Until further study can clarify the relationships of these species, and considering the geographic isolation of this group, we assign all of the species of Eleutherodactylus in southeastern Brazil (excluding E. binotatus and E. plicifer) to the genus Ischnocnema.

For the large South American Clade, we resurrect the name Pristimantis Jiménez de la Espada, 1870 (type species, Pristimantis galdi). P. galdi has been placed in the unistrigatus group of Eleutherodactylus (10), a species group that contains species that appear in the South American Clade in our analyses. After this study was completed, we obtained sequence of this species and have confirmed that it is highly nested in the South American Clade. For the clade encompassing members of the anomalus, bufoniformis, and sulcatus groups, we apply the available name Limnophys Jiménez de la Espada, 1871 (type species, Limnophys cornutus). The clade including members of the dolops and nigrovittatus groups, as well as some species of Phrynopus, requires a new generic name, which will be described elsewhere. Further details on all of these taxonomic decisions will be treated elsewhere by us in a monograph on the classification of this complex of >800 species.

Geographic Distributions of Major Clades. Although the three major clades (the Caribbean Clade, the Middle American Clade, and the South American Clade) are strongly associated with geography, each includes species that occur in other areas. In the Caribbean Clade, 24 members of the subgenus Syrrhophus (all except Eleutherodactylus zeus and E. symingtoni) occur in North and Middle America. In the Middle American Clade, five species (Craugastor crassidigitus, C. fitzingeri, C. longirostris, C. opimus, and C. raniformis) occur in both Central and South America, and one (C. biporcatus) is endemic to South America. In the South American Clade, two species are endemic to the Lesser Antilles (Pristimantis euphronides and P. shrevei), nine are endemic to southern Central America (P. altae, P. cerasinus, P. diastema, P. hylaeformis, P. laticorpus, P. museosus, P. pardalis, P. pirrensis, and P. tigrillo), and nine are found in Central and South America (P. achatinus, P. caryophyllaceus, P. cruentus, P. gaigeae, P. moro, P. quidditus, P. ridens, P. taeniatus, and P. vocator). It can be assumed that those species of Craugastor and Pristimantis occurring in both Central and South America achieved their current distributions through Pliocene or later overland dispersal. However, the possibility of over-water dispersal for C. biporcatus or endemic Central American Pristimantis cannot be excluded. Our data set includes none of these endemics, so no determination based on times of divergence can be made. Thus, recent (<3 Mya) speciation or overland dispersal, followed by source-area extinction, or earlier over-water dispersal all remain possible explanations for the current distributions of these species.

Data Collection. In addition to broadly sampling the eleutherodactyline genera, our data set also spans a broad geographic range. Included were seven eleutherodactyline species from Southeast Brazil (plus one species of Brachycephalus), 116 from other parts of South America, 19 Middle American species, and 140 West Indian species. These regions respectively contain totals of 47, 477, 154, and 149 described species.

Tissue samples were hand-collected by using approved methods (Pennsylvania State University Institutional Animal Care and Use Committee approval 17632, for those collected by S.B.H.). They were frozen in liquid nitrogen or preserved in ethanol and kept cold during transport. Additional ethanol-preserved or frozen tissue samples were obtained from museum sources or other researchers. In the laboratory, samples were maintained at -80°C. In addition to sequences generated from these tissues, additional sequences were obtained from GenBank. SI Table 4 lists all individual specimens used in this study, including source, tissue collection number (if applicable), museum voucher number (if available), genes sampled, and corresponding GenBank accession numbers for each sequence. We chose the 12S and 16S genes because of their slower rate of evolution, as compared to other mitochondrial genes, in an attempt to avoid saturation problems (multiple nucleotide substitutions at the same site). The fragment of nuclear gene Tyr was chosen because it has proven informative in other anuran studies (2, 11). The fragment of nuclear gene Rag-1 is from the relatively faster-evolving first half of the gene.

Genomic DNA was extracted using the Qiagen DNeasy tissue extraction kit under the manufacturer's protocol. PCR amplification of samples was performed in 50-ml reactions using AmpliTaq DNA polymerase and ThermoPol buffer (New England Biolabs). For amplification of mitochondrial genes, each reaction contained ThermoPol buffer at 1×, dNTPs at 4 mM, forward and reverse primers at 1 mM, one unit of polymerase, and 1 ml of extracted DNA (more for low-quality tissue). For amplification of nuclear genes, dNTP was increased to 6.6 mM, polymerase to 2.5 units, and extracted DNA to 5 ml. Standard reaction conditions were an initial hold for 5 min at 94°C, followed by 40 cycles of 94°C for 30 s, 50°C for 30 s, and 72°C for 60 s. After 40 cycles, a final hold of 72°C for 7 min was performed before terminating the reaction at 4°C. For low- or nonyielding samples, annealing temperature was dropped from 50°C to 46°C. Primers used in PCR reactions were obtained from the literature or designed in the lab (see SI Table 5). Amplified PCR products were isolated by running on agarose gels and filtering with Millipore Ultrafree-DA gel filters or by vacuum filtration using Millipore Multiscreen filters.

Cycle sequencing was performed by using either the Amersham Pharmacia DYEnamic ET terminator cycle sequencing kit or ABI BigDye terminator cycle sequencing kit under manufacturers' guidelines. DNA sequencing was performed with an ABI 3100-Avant or 3730 genetic analyzer. Cycle sequencing and analysis were performed by the authors or by the Pennsylvania State University Nucleic Acid Facility. All fragments were sequenced in both forward and reverse directions.

Before analyses involving any sequence, all chromatograms were fully inspected, and all sequences were compared against their reverse complement to detect any call errors. Embedded primer sequences were deleted from all sequence fragments before assembly or alignment. Alignments of 12S and 16S sequences were created by using CLUSTAL X under default parameters. Resulting alignments were inspected for errors and compared against secondary structure models available from the European ribosomal RNA database. Regions of uncertain homology were excluded from analysis. Sequences for Rag-1 and Tyr were aligned by eye.

Phylogenetic Analyses. In certain cases, species deemed important to the study were missing large parts of data for one gene. This was due in part to the lack of available tissues or GenBank sequences and in part to the inability to amplify or sequence certain regions. In these instances, the available sequences were used, and unknown regions were coded as missing data. Additionally, 7 species in the 65-species data set were missing data for one of the two nuclear genes (SI Table 4). In the case of Limnophys anomalus, the sequence of the closely related species L. bufoniformis was substituted for the Tyr fragment because L. anomalus was the only species of Limnophys in the 65 species data set and Tyr is the least variable gene in our study.

Model choice for likelihood and minimum evolution analyses were based partly on the limitations of the software packages used. Our preferred model for all analyses was GTR + I + G. However, neither RAxML nor MEGA can employ this model. Therefore, substitute models available in each package close to the GTR + I + G model were chosen for likelihood and minimum evolution analyses.

Bayesian analyses were run for 20,000,000 (280-species), 10,000,000 (146-species), or 2,000,000 (65-species) generations with three heated and one cold chain. Chains were sampled every 1,000, 500, or 100 generations, respectively. The first 25% of samples were discarded as burn-in. To ensure that this was an adequate number of samples discarded for each analysis, plots of log likelihood vs. generation were produced for every Bayesian analysis. In all cases, the region of increasing log likelihood values was encompassed in the first 25% of samples. Convergence for each Bayesian analysis was assessed by using the program Tracer 1.3 (12) to obtain estimated sample sizes for each model parameter (six substitution frequency categories, four nucleotide frequency categories, g-parameter, proportion of invariant sites, tree length, and log likelihood). Estimated sample sizes of each parameter were >100 for both independent runs of nearly all analyses, except for a substitution frequency (ESS = 99) and a nucleotide frequency parameter (ESS = 90) for one of the 146-species runs, the tree length parameter (ESS = 98) for the other 146-species run, and the tree length parameter (ESS = 94) for one run of the 65-species nuclear + mitochondrial data set. All ESS values were >200 when the two independent runs of each analysis were combined.

For the 65-species dataset, ME, ML, and Bayesian analyses were run with the nuclear gene data set separately (data not shown). The same major clades appeared in all of these analyses.

Divergence Time Estimation. Geologic times and boundaries of periods used here are from a recent update (13). The five chosen calibrations were based on several independent lines of evidence. Jamaica did not become permanently emergent until 10 Mya (14, 15), setting a maximum time for basal divergences in the Jamaican clade (including representative species E. gossei and E. luteolus). The Hispaniolan South Island has a similar geologic history (16), setting a maximum time of 10 Mya for the basal divergence in the South Island clade (e.g., between representative species E. thorectes and E. caribe). An Eleutherodactylus fossil in amber from northern Hispaniola (17) is dated 15-20 Mya (18). The fossil is assumed to be in the subgenus Eleutherodactylus based on location (North Island of Hispaniola), age (older than uplift of South Island), and normal head width as compared with members of the subgenus Pelorius (which are wide-headed) (19). This establishes a minimum date for the origin of the lineage leading to the subgenus Eleutherodactylus on Hispaniola (divergence of the subgenera Pelorius and Eleutherodactylus). Australian hylids (pelodryadines) are most closely related to South American hylids (phyllomedusines), and probably arrived by overland dispersal via Antarctica (20, 21). The time window for this dispersal was 35-70 Mya (22-25), providing constraints for the divergence of representatives Litoria caerulea and Agalychnis callidryas.

Multidivtime requires prior estimates for rttm, rtsd, rtrate, rtratesd, brownmean, brownsd, and bigtime. The prior for the rttm (in-group root) parameter was set at 65 Mya based on recent data (26) that place hyloid family divergences near the K-T boundary. Rttmsd, the standard deviation of rttm, was set at 25 Mya. This is a conservative estimate, considering that the true date of the ingroup root is almost certainly less than one standard deviation from rttm in either direction. Higher values of rttm (80 Mya) and rttmsd (40 Mya) were also used to test their results on timing analyses. The prior for rtrate was set at 0.0075 for the mt analysis, 0.0015 for the nuclear analysis, and 0.0035 for the combined analysis. These values were obtained by first dividing the typical root-to-tip branch length for each gene (as determined by estbranches in the Multidivtime package) by rttm, and then taking the average of these values for the genes used in each respective analysis. As a conservative measure, the prior for rtratesd was set equal to rtrate in each analysis. The prior for brownmean was set at 0.0125, arrived at by dividing one by rttm. The prior for brownsd was set equal to brownmean because of the large measure of uncertainty in the prior for brownmean. Bigtime was set at 150 Mya. All other parameters (minab, newk, othk, thek) were maintained at default values. Analyses were run for 1,100,000 generations, with a sample frequency of 100 after a burn-in of 100,000 generations.

In addition to the times presented (SI Table 3), analyses were also performed with higher values for the rttm and rttmsd priors (see SI Text). Changing these priors had little effect on resulting dates, which differed by <3% when both rttm and rttmsd are increased, and by even less if only one of the priors was increased.

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Table 2. Taxonomic listing of all eleutherodactylines and Brachycephalus (812 sp.)

"Eleutherodactylus" binotatus*

Spix, 1824

"Eleutherodactylus" plicifer*

Boulenger, 1888

"Eleutherodactylus" cruralis

Boulenger, 1902

"Eleutherodactylus" discoidalis

Peracca, 1895

"Eleutherodactylus" heterodactylus

Miranda-Ribeiro, 1937

"Eleutherodactylus" ibischi

Reichle, Lötters and de la Riva, 2001

"Eleutherodactylus" madidi

Padial, Gonzáles, and De la Riva, 2005

"Eleutherodactylus" pereger

Lynch, 1975

"Eleutherodactylus" zongoensis

Reichle and Köhler, 1997

"Eleutherodactylus" adercus

Lynch, 2003

"Eleutherodactylus" araiodactylus

Duellman and Pramuk, 1999

"Eleutherodactylus" babax

Lynch, 1989

"Eleutherodactylus" dolops

Lynch and Duellman, 1980

"Eleutherodactylus" elassodiscus

Lynch, 1973

"Eleutherodactylus" fallaciosus

Duellman, 2000

"Eleutherodactylus" flavomaculatus

Parker, 1938

"Eleutherodactylus" latens

Lynch, 1989

"Eleutherodactylus" lucida

Cannatella, 1984

"Eleutherodactylus" lundbergi

Lehr, 2005

"Eleutherodactylus" mantipus

Boulenger, 1908

"Eleutherodactylus" nebulanastes

Cannatella, 1984

"Eleutherodactylus" nigrovittatus

Andersson, 1945

"Phrynopus" brunneus

Lynch, 1975

"Phrynopus" peraccai

Lynch, 1975

Adelophryne adiastola

Hoogmoed and Lescure, 1984

Adelophryne baturitensis

Hoogmoed, Borges, and Cascon, 1994

Adelophryne gutturosa

Hoogmoed and Lescure, 1984

Adelophryne maranguapensis

Hoogmoed, Borges, and Cascon, 1994

Adelophryne pachydactyla

Hoogmoed, Borges, and Cascon, 1994

Atopophrynus syntomopus

Lynch and Ruiz-Carranza, 1982

Barycholos pulcher

Boulenger, 1898

Barycholos ternetzi

Miranda-Ribeiro, 1937

Brachycephalus alipioi

Pombal and Gasparini, 2006

Brachycephalus brunneus

Ribero, Alves, Haddad, and Dos Reis, 2005

Brachycephalus didactylus

Izecksohn, 1971

Brachycephalus ephippium

Spix, 1824

Brachycephalus ferruginus

Alves, Ribeiro, Haddad, and Dos Reis, 2006

Brachycephalus hermogenesi

Giaretta and Sawaya, 1998

Brachycephalus izecksohni

Ribero, Alves, Haddad, and Dos Reis, 2005

Brachycephalus nodoterga

Miranda-Ribeiro, 1920

Brachycephalus pernix

Pombal, Wistuba, and Bornschein, 1998

Brachycephalus pombali

Alves, Ribeiro, Haddad, and Dos Reis, 2006

Brachycephalus vertebralis

Pombal, 2001

Craugastor adamastus

Campbell, 1994

Craugastor alfredi

Boulenger, 1898

Craugastor amniscola

Campbell and Savage, 2000

Craugastor anciano

Savage, McCranie and Wilson, 1988

Craugastor andi

Savage, 1974

Craugastor angelicus

Savage, 1975

Craugastor aphanus

Campbell, 1994

Craugastor augusti

Dugès, 1879

Craugastor aurilegulus

Savage, McCranie and Wilson, 1988

Craugastor azueroensis

Savage, 1975

Craugastor batrachylus

Taylor, 1940

Craugastor berkenbuschii

Peters, 1870

Craugastor biporcatus

Peters, 1863

Craugastor bocourti

Brocchi, 1877

Craugastor bransfordii

Cope, 1886

Craugastor brocchi

Boulenger, 1882

Craugastor campbelli

Smith, 2005

Craugastor catalinae

Campbell and Savage, 2000

Craugastor chac

Savage, 1987

Craugastor charadra

Campbell and Savage, 2000

Craugastor chrysozetetes

McCranie, Savage and Wilson, 1989

Craugastor coffeus

McCranie and Köhler, 1999

Craugastor crassidigitus

Taylor, 1952

Craugastor cruzi

McCranie, Savage and Wilson, 1989

Craugastor cuaquero

Savage, 1980

Craugastor cyanochthebius

McCranie and Smith, 2006

Craugastor daryi

Ford and Savage, 1984

Craugastor decoratus

Taylor, 1942

Craugastor emcelae

Lynch, 1985

Craugastor emleni

Dunn and Emlen, 1932

Craugastor epochthidius

McCranie and Wilson, 1997

Craugastor escoces

Savage, 1975

Craugastor fecundus

McCranie and Wilson, 1997

Craugastor fitzingeri

Schmidt, 1857

Craugastor fleischmanni

Boettger, 1892

Craugastor galacticorhinus

Canseco-Márquez and Smith, 2004

Craugastor glaucus

Lynch, 1967

Craugastor gollmeri

Peters, 1863

Craugastor greggi

Bumzahem, 1955

Craugastor guerreroensis

Lynch, 1967

Craugastor gulosus

Cope, 1875

Craugastor hobartsmithi

Taylor, 1937

Craugastor inachus

Campbell and Savage, 2000

Craugastor jota

Lynch, 1980

Craugastor laevissimus

Werner, 1896

Craugastor laticeps

Duméril, 1853

Craugastor lauraster

Savage, McCranie and Espinal, 1996

Craugastor lineatus

Brocchi, 1879

Craugastor loki

Shannon and Werler, 1955

Craugastor longirostris

Boulenger, 1898

Craugastor matudai

Taylor, 1941

Craugastor megacephalus

Cope, 1875

Craugastor megalotympanum

Shannon and Werler, 1955

Craugastor melanostictus

Cope, 1875

Craugastor merendonensis

Schmidt, 1933

Craugastor mexicanus

Brocchi, 1877

Craugastor milesi

Schmidt, 1933

Craugastor mimus

Taylor, 1955

Craugastor monnichorum

Dunn, 1940

Craugastor montanus

Lynch, 1965

Craugastor myllomyllon

Savage, 2000

Craugastor nefrens

Smith, 2005

Craugastor noblei

Barbour and Dunn, 1921

Craugastor obesus

Barbour, 1928

Craugastor occidentalis

Taylor, 1941

Craugastor olanchano

McCranie and Wilson, 1999

Craugastor omiltemanus

Günther, 1900

Craugastor omoaensis

McCranie and Wilson, 1997

Craugastor opimus

Savage and Myers, 2002

Craugastor palenque

Campbell and Savage, 2000

Craugastor pechorum

McCranie and Wilson, 1999

Craugastor pelorus

Campbell and Savage, 2000

Craugastor persimilis

Barbour, 1926

Craugastor phasma

Lips and Savage, 1996

Craugastor podiciferus

Cope, 1875

Craugastor polymniae

Campbell, Lamar and Hillis, 1989

Craugastor polyptychus

Cope, 1886

Craugastor pozo

Johnson and Savage, 1995

Craugastor psephosypharus

Campbell, Savage and Meyer, 1994

Craugastor punctariolus

Peters, 1863

Craugastor pygmaeus

Taylor, 1937

Craugastor raniformis

Boulenger, 1896

Craugastor ranoides

Cope, 1886

Craugastor rayo

Savage and DeWeese, 1979

Craugastor rhodopis

Cope, 1867

Craugastor rhyacobatrachus

Campbell and Savage, 2000

Craugastor rivulus

Campbell and Savage, 2000

Craugastor rostralis

Werner, 1896

Craugastor rugosus

Peters, 1873

Craugastor rugulosus

Cope, 1870

Craugastor rupinius

Campbell and Savage, 2000

Craugastor sabrinus

Campbell and Savage, 2000

Craugastor saltuarius

McCranie and Wilson, 1997

Craugastor sandersoni

Schmidt, 1941

Craugastor silvicola

Lynch, 1967

Craugastor spatulatus

Smith, 1939

Craugastor stadelmani

Schmidt, 1936

Craugastor stejnegerianus

Cope, 1893

Craugastor stuarti

Lynch, 1967

Craugastor tabasarae

Savage, Hollingsworth, Lips, and Jaslow, 2004

Craugastor talamancae

Dunn, 1931

Craugastor tarahumaraensis

Taylor, 1940

Craugastor taurus

Taylor, 1958

Craugastor taylori

Lynch, 1966

Craugastor trachydermus

Campbell, 1994

Craugastor underwoodi

Boulenger, 1896

Craugastor uno

Savage, 1984

Craugastor vocalis

Taylor, 1940

Craugastor vulcani

Shannon and Werler, 1955

Craugastor xucanebi

Stuart, 1941

Craugastor yucatanensis

Lynch, 1965

Dischidodactylus colonnelloi

Ayarzagüena, 1985

Dischidodactylus duidensis

Rivero, 1968

Eleutherodactylus (Eleutherodactylus) abbotti

Cochran, 1923

Eleutherodactylus (Eleutherodactylus) amplinympha

Kaiser, Green and Schmid, 1994

Eleutherodactylus (Eleutherodactylus) antillensis

Reinhardt and Lütken, 1863

Eleutherodactylus (Eleutherodactylus) audanti

Cochran, 1934

Eleutherodactylus (Eleutherodactylus) auriculatoides

Noble, 1923

Eleutherodactylus (Eleutherodactylus) auriculatus

Cope, 1862

Eleutherodactylus (Eleutherodactylus) barlagnei

Lynch, 1965

Eleutherodactylus (Eleutherodactylus) bartonsmithi

Schwartz, 1960

Eleutherodactylus (Eleutherodactylus) brittoni

Schmidt, 1920

Eleutherodactylus (Eleutherodactylus) cochranae

Grant, 1932

Eleutherodactylus (Eleutherodactylus) cooki

Grant, 1932

Eleutherodactylus (Eleutherodactylus) coqui

Thomas, 1966

Eleutherodactylus (Eleutherodactylus) eileenae

Dunn, 1926

Eleutherodactylus (Eleutherodactylus) eneidae

Rivero, 1959

Eleutherodactylus (Eleutherodactylus) flavescens

Noble, 1923

Eleutherodactylus (Eleutherodactylus) fowleri

Schwartz, 1973

Eleutherodactylus (Eleutherodactylus) glamyrus

Estrada and Hedges, 1997

Eleutherodactylus (Eleutherodactylus) gryllus

Schmidt, 1920

Eleutherodactylus (Eleutherodactylus) guantanamera

Hedges, Estrada and Thomas, 1992

Eleutherodactylus (Eleutherodactylus) haitianus

Barbour, 1942

Eleutherodactylus (Eleutherodactylus) hedricki

Rivero, 1963

Eleutherodactylus (Eleutherodactylus) ionthus

Schwartz, 1960

Eleutherodactylus (Eleutherodactylus) jasperi

Drewry and Jones, 1976

Eleutherodactylus (Eleutherodactylus) johnstonei

Barbour, 1914

Eleutherodactylus (Eleutherodactylus) karlschmidti

Grant, 1931

Eleutherodactylus (Eleutherodactylus) lamprotes

Schwartz, 1973

Eleutherodactylus (Eleutherodactylus) leberi

Schwartz, 1965

Eleutherodactylus (Eleutherodactylus) locustus

Schmidt, 1920

Eleutherodactylus (Eleutherodactylus) mariposa

Hedges, Estrada and Thomas, 1992

Eleutherodactylus (Eleutherodactylus) martinicensis

Tschudi, 1838

Eleutherodactylus (Eleutherodactylus) melacara

Hedges, Estrada and Thomas, 1992

Eleutherodactylus (Eleutherodactylus) minutus

Noble, 1923

Eleutherodactylus (Eleutherodactylus) montanus

Schmidt, 1919

Eleutherodactylus (Eleutherodactylus) parabates

Schwartz, 1964

Eleutherodactylus (Eleutherodactylus) patriciae

Schwartz, 1965

Eleutherodactylus (Eleutherodactylus) pinchoni

Schwartz, 1967

Eleutherodactylus (Eleutherodactylus) pituinus

Schwartz, 1965

Eleutherodactylus (Eleutherodactylus) poolei

Cochran, 1938

Eleutherodactylus (Eleutherodactylus) portoricensis

Schmidt, 1927

Eleutherodactylus (Eleutherodactylus) principalis

Estrada and Hedges, 1997

Eleutherodactylus (Eleutherodactylus) richmondi

Stejneger, 1904

Eleutherodactylus (Eleutherodactylus) ronaldi

Schwartz, 1960

Eleutherodactylus (Eleutherodactylus) schwartzi

Thomas, 1966

Eleutherodactylus (Eleutherodactylus) unicolor

Stejneger, 1904

Eleutherodactylus (Eleutherodactylus) varians

Gundlach and Peters, 1864

Eleutherodactylus (Eleutherodactylus) wetmorei

Cochran, 1932

Eleutherodactylus (Eleutherodactylus) wightmanae

Schmidt, 1920

Eleutherodactylus (Euhyas) acmonis

Schwartz, 1960

Eleutherodactylus (Euhyas) adelus

Diaz, Cadiz and Hedges, 2003

Eleutherodactylus (Euhyas) albipes

Barbour and Shreve, 1937

Eleutherodactylus (Euhyas) alcoae

Schwartz, 1971

Eleutherodactylus (Euhyas) alticola

Lynn, 1937

Eleutherodactylus (Euhyas) amadeus

Hedges, Thomas and Franz, 1987

Eleutherodactylus (Euhyas) andrewsi

Lynn, 1937

Eleutherodactylus (Euhyas) apostates

Schwartz, 1973

Eleutherodactylus (Euhyas) armstrongi

Noble and Hassler, 1933

Eleutherodactylus (Euhyas) atkinsi

Dunn, 1925

Eleutherodactylus (Euhyas) bakeri

Cochran, 1935

Eleutherodactylus (Euhyas) blairhedgesi

Estrada, Diaz and Rodriguez, 1998

Eleutherodactylus (Euhyas) bresslerae

Schwartz, 1960

Eleutherodactylus (Euhyas) brevirostris

Shreve, 1936

Eleutherodactylus (Euhyas) caribe

Hedges and Thomas, 1992

Eleutherodactylus (Euhyas) casparii

Dunn, 1926

Eleutherodactylus (Euhyas) cavernicola

Lynn, 1954

Eleutherodactylus (Euhyas) corona

Hedges and Thomas, 1992

Eleutherodactylus (Euhyas) cubanus

Barbour, 1942

Eleutherodactylus (Euhyas) cundalli

Dunn, 1926

Eleutherodactylus (Euhyas) cuneatus

Cope, 1862

Eleutherodactylus (Euhyas) darlingtoni

Cochran, 1935

Eleutherodactylus (Euhyas) dimidiatus

Cope, 1862

Eleutherodactylus (Euhyas) dolomedes

Hedges and Thomas, 1992

Eleutherodactylus (Euhyas) emiliae

Dunn, 1926

Eleutherodactylus (Euhyas) etheridgei

Schwartz, 1958

Eleutherodactylus (Euhyas) eunaster

Schwartz, 1973

Eleutherodactylus (Euhyas) furcyensis

Shreve and Williams, 1963

Eleutherodactylus (Euhyas) fuscus

Lynn and Dent, 1943

Eleutherodactylus (Euhyas) glandulifer

Cochran, 1935

Eleutherodactylus (Euhyas) glanduliferoides

Shreve, 1936

Eleutherodactylus (Euhyas) glaphycompus

Schwartz, 1973

Eleutherodactylus (Euhyas) glaucoreius

Schwartz and Fowler, 1973

Eleutherodactylus (Euhyas) goini

Schwartz, 1960

Eleutherodactylus (Euhyas) gossei

Dunn, 1926

Eleutherodactylus (Euhyas) grabhami

Dunn, 1926

Eleutherodactylus (Euhyas) grahami

Schwartz, 1979

Eleutherodactylus (Euhyas) greyi

Dunn, 1926

Eleutherodactylus (Euhyas) griphus

Crombie, 1986

Eleutherodactylus (Euhyas) guanahacabibes

Estrada and Rodriguez, 1985

Eleutherodactylus (Euhyas) gundlachi

Schmidt, 1920

Eleutherodactylus (Euhyas) heminota

Shreve and Williams, 1963

Eleutherodactylus (Euhyas) iberia

Estrada and Hedges, 1996

Eleutherodactylus (Euhyas) intermedius

Barbour and Shreve, 1937

Eleutherodactylus (Euhyas) jamaicensis

Barbour, 1910

Eleutherodactylus (Euhyas) jaumei

Estrada and Alonso, 1997

Eleutherodactylus (Euhyas) jugans

Cochran, 1937

Eleutherodactylus (Euhyas) junori

Dunn, 1926

Eleutherodactylus (Euhyas) klinikowskii

Schwartz, 1959

Eleutherodactylus (Euhyas) lentus

Cope, 1862

Eleutherodactylus (Euhyas) leoncei

Shreve and Williams, 1963

Eleutherodactylus (Euhyas) limbatus

Cope, 1862

Eleutherodactylus (Euhyas) lucioi

Schwartz, 1980

Eleutherodactylus (Euhyas) luteolus

Gosse, 1851

Eleutherodactylus (Euhyas) maestrensis

Díaz, Cádiz, and Navarro, 2005

Eleutherodactylus (Euhyas) michaelschmidi

Díaz, Cádiz, and Navarro, 2007

Eleutherodactylus (Euhyas) monensis

Meerwarth, 1901

Eleutherodactylus (Euhyas) nubicola

Dunn, 1926

Eleutherodactylus (Euhyas) orcutti

Dunn, 1928

Eleutherodactylus (Euhyas) orientalis

Barbour and Shreve, 1973

Eleutherodactylus (Euhyas) oxyrhyncus

Duméril and Bibron, 1841

Eleutherodactylus (Euhyas) pantoni

Dunn, 1926

Eleutherodactylus (Euhyas) paulsoni

Schwartz, 1964

Eleutherodactylus (Euhyas) pentasyringos

Schwartz and Fowler, 1973

Eleutherodactylus (Euhyas) pezopetrus

Schwartz, 1960

Eleutherodactylus (Euhyas) pictissimus

Cochran, 1935

Eleutherodactylus (Euhyas) pinarensis

Dunn, 1926

Eleutherodactylus (Euhyas) planirostris

Cope, 1862

Eleutherodactylus (Euhyas) probolaeus

Schwartz, 1965

Eleutherodactylus (Euhyas) rhodesi

Schwartz, 1980

Eleutherodactylus (Euhyas) ricordii

Duméril and Bibron, 1841

Eleutherodactylus (Euhyas) riparius

Estrada and Hedges, 1998

Eleutherodactylus (Euhyas) rivularis

Diaz, Estrada and Hedges, 2001

Eleutherodactylus (Euhyas) rogersi

Goin, 1955

Eleutherodactylus (Euhyas) rufifemoralis

Noble and Hassler, 1933

Eleutherodactylus (Euhyas) schmidti

Noble, 1923

Eleutherodactylus (Euhyas) sciagraphus

Schwartz, 1973

Eleutherodactylus (Euhyas) semipalmatus

Shreve, 1936

Eleutherodactylus (Euhyas) simulans

Díaz and Fong, 2001

Eleutherodactylus (Euhyas) sisyphodemus

Crombie, 1977

Eleutherodactylus (Euhyas) tetajulia

Estrada and Hedges, 1996

Eleutherodactylus (Euhyas) thomasi

Schwartz, 1959

Eleutherodactylus (Euhyas) thorectes

Hedges, 1988

Eleutherodactylus (Euhyas) toa

Estrada and Hedges, 1991

Eleutherodactylus (Euhyas) tonyi

Estrada and Hedges, 1997

Eleutherodactylus (Euhyas) turquinensis

Barbour and Shreve, 1937

Eleutherodactylus (Euhyas) varleyi

Dunn, 1925

Eleutherodactylus (Euhyas) ventrilineatus

Shreve, 1936

Eleutherodactylus (Euhyas) warreni

Schwartz, 1976

Eleutherodactylus (Euhyas) weinlandi

Barbour, 1914

Eleutherodactylus (Euhyas) zugi

Schwartz, 1958

Eleutherodactylus (Pelorius) chlorophenax

Schwartz, 1976

Eleutherodactylus (Pelorius) hypostenor

Schwartz, 1965

Eleutherodactylus (Pelorius) inoptatus

Barbour, 1914

Eleutherodactylus (Pelorius) nortoni

Schwartz, 1976

Eleutherodactylus (Pelorius) parapelates

Hedges and Thomas, 1987

Eleutherodactylus (Pelorius) ruthae

Noble, 1923

Eleutherodactylus (Syrrhophus) albolabris

Lynch, 1991

Eleutherodactylus (Syrrhophus) angustidigitorum

Taylor, 1940

Eleutherodactylus (Syrrhophus) cystignathoides

Cope, 1817

Eleutherodactylus (Syrrhophus) dennisi

Lynch, 1970

Eleutherodactylus (Syrrhophus) dilatus

Davis and Dixon, 1955

Eleutherodactylus (Syrrhophus) grandis

Dixon, 1957

Eleutherodactylus (Syrrhophus) guttilatus

Cope, 1979

Eleutherodactylus (Syrrhophus) interorbitalis

Langebartel and Shannon, 1956

Eleutherodactylus (Syrrhophus) leprus

Cope, 1879

Eleutherodactylus (Syrrhophus) longipes

Baird, 1859

Eleutherodactylus (Syrrhophus) marnockii

Cope, 1878

Eleutherodactylus (Syrrhophus) maurus

Hedges, 1989

Eleutherodactylus (Syrrhophus) modestus

Taylor, 1942

Eleutherodactylus (Syrrhophus) nitidus

Peters, 1870

Eleutherodactylus (Syrrhophus) nivicolimae

Dixon and Webb, 1996

Eleutherodactylus (Syrrhophus) pallidus

Duellman, 1958

Eleutherodactylus (Syrrhophus) pipilans

Taylor, 1940

Eleutherodactylus (Syrrhophus) rubrimaculatus

Taylor and Smith, 1945

Eleutherodactylus (Syrrhophus) rufescens

Duellman and Dixon, 1959

Eleutherodactylus (Syrrhophus) saxatilis

Webb, 1962

Eleutherodactylus (Syrrhophus) symingtoni

Schwartz, 1957

Eleutherodactylus (Syrrhophus) syristes

Hoyt, 1965

Eleutherodactylus (Syrrhophus) teretistes

Duellman, 1958

Eleutherodactylus (Syrrhophus) verrucipes

Cope, 1885

Eleutherodactylus (Syrrhophus) verruculatus

Peters, 1870

Eleutherodactylus (Syrrhophus) zeus

Schwartz, 1958

Eleutherodactylus counouspeus

Schwartz, 1964

Euparkerella brasiliensis

Parker, 1926

Euparkerella cochranae

Izecksohn, 1988

Euparkerella robusta

Izecksohn, 1988

Euparkerella tridactyla

Izecksohn, 1988

Geobatrachus walkeri

Ruthveni, 1915

Holoaden bradei

Lutz, 1958

Holoaden luederwaldti

Miranda-Ribero, 1920

Ischnocnema bilineata

Bokermann, 1975

Ischnocnema bolbodactyla

Lutz, 1925

Ischnocnema epipeda

Heyer, 1984

Ischnocnema erythromera

Heyer, 1984

Ischnocnema gehrti

Miranda-Ribeiro, 1926

Ischnocnema gualteri

Lutz, 1974

Ischnocnema guentheri

Steindachner, 1864

Ischnocnema henselii

Peters, 1870

Ischnocnema hoehnei

Lutz, 1958

Ischnocnema holti

Cochran, 1948

Ischnocnema izecksohni

Caramaschi and Kisteumacher, 1989

Ischnocnema juipoca

Sazima and Cardoso, 1978

Ischnocnema lactea

Miranda-Ribeiro, 1923

Ischnocnema manezinho

Garcia, 1996

Ischnocnema nasuta

Lutz, 1925

Ischnocnema nigriventris

Lutz, 1925

Ischnocnema octavioi

Bokermann, 1965

Ischnocnema oeus

Heyer, 1984

Ischnocnema paranaensis

Langone and Segalla, 1996

Ischnocnema parva

Girard, 1853

Ischnocnema paulodutrai

Bokermann, 1975

Ischnocnema pusilla

Bokermann, 1967

Ischnocnema ramagii

Boulenger, 1888

Ischnocnema randorum

Heyer, 1985

Ischnocnema sambaqui

Castanho and Haddad, 2000

Ischnocnema spanios

Heyer, 1985

Ischnocnema venancioi

Lutz, 1958

Ischnocnema verrucosa

Reinhardt and Lütken, 1862

Ischnocnema vinhai

Bokermann, 1975

Limnophys anatipes

Lynch and Myers, 1983

Limnophys anomalus

Boulenger, 1898

Limnophys bufoniformis

Boulenger, 1896

Limnophys cadenai

Lynch, 1986

Limnophys cerastes

Lynch, 1975

Limnophys cheiroplethus

Lynch, 1990

Limnophys cornutus

Jiménez de la Espada, 1871

Limnophys helonotus

Lynch, 1975

Limnophys ingeri

Cochran and Goin, 1961

Limnophys laticorpus

Myers and Lynch, 1997

Limnophys necerus

Lynch, 1975

Limnophys necopinus

Lynch, 1997

Limnophys ruizi

Lynch, 1981

Limnophys sulcatus

Cope, 1874

Limnophys zygodactylus

Lynch and Myers, 1983

Oreobates choristolemma

Harvey and Sheehy, 2005

Oreobates quixensis

Jiménez de la Espada, 1872

Oreobates sanctaecrucis

Harvey and Keck, 1995

Oreobates sanderi

Padial, Reichle, and De la Riva

Oreobates saxatilis

Duellman, 1990

Oreobates simmonsi

Padial, Reichle, and De La Riva, 2005

Phrynopus adenobrachius

Ardila-Robayo, Ruiz-Carranza, and Barrera-Rodriguez, 1996

Phrynopus adenopleurus

Aguayo-Vedia and Harvey, 2001

Phrynopus bagrecitoi

Lynch, 1986

Phrynopus barthlenae

Lehr and Aguilar, 2002

Phrynopus boettgeri

Lehr, 2006

Phrynopus bracki

Hedges, 1990

Phrynopus bufoides

Lehr, Lundberg, and Aguilar, 2005

Phrynopus columbianus

Werner, 1899

Phrynopus cophites

Lynch, 1975

Phrynopus dagmarae

Lehr, Aguilar, and Köhler, 2002

Phrynopus heimorum

Lehr, 2001

Phrynopus horstpauli

Lehr, Köhler, and Ponce, 2000

Phrynopus iatamasi

Aguayo-Vedia and Harvey, 2001

Phrynopus juninensis

Shreve, 1938

Phrynopus kauneorum

Lehr, Aguilar, and Köhler, 2002

Phrynopus kempffi

De la Riva, 1992

Phrynopus montium

Shreve, 1938

Phrynopus nanus

Goin and Cochran, 1963

Phrynopus parkeri

Lynch, 1975

Phrynopus paucari

Lehr, Lundberg, and Aguilar,2005

Phrynopus peruanus

Peters, 1873

Phrynopus peruvianus

Noble, 1921

Phrynopus pesantesi

Lehr, Lundberg, and Aguilar, 2005

Phrynopus pinguis

Harvey and Ergueta, 1998

Phrynopus tautzorum

Lehr and Aguilar, 2003

Phrynopus thompsoni

Duellman, 2000

Phrynopus wettsteini

Parker, 1932

Phyllonastes carrascoicola

De la Riva and Köhler, 1998

Phyllonastes duellmani

Lehr, Aguilar, and Lundberg, 2004

Phyllonastes heyeri

Lynch, 1986

Phyllonastes lochites

Lynch, 1976

Phyllonastes lynchi

Duellman, 1991

Phyllonastes myrmecoides

Lynch, 1976

Phyllonastes ritarasquinae

Köhler, 2000

Phyzelaphryne miriamae

Heyer, 1977

Pristimantis aaptus

Lynch and Lescure, 1980

Pristimantis acatallelus

Lynch and Ruíz-Carranza, 1983

Pristimantis acerus

Lynch and Duellman, 1980

Pristimantis achatinus

Boulenger, 1898

Pristimantis actinolaimus

Lynch and Rueda-Almonacid, 1998

Pristimantis actites

Lynch, 1979

Pristimantis acuminatus

Shreve, 1935

Pristimantis acutirostris

Lynch, 1984

Pristimantis aemulatus

Ruíz-Carranza, Lynch and Ardila-Robayo, 1997

Pristimantis affinis

Werner, 1899

Pristimantis alalocophus

Roa-Trujillo and Ruíz-Carranza, 1991

Pristimantis albericoi

Lynch and Ruíz-Carranza, 1996

Pristimantis altae

Dunn, 1942

Pristimantis altamazonicus

Barbour and Dunn, 1921

Pristimantis anemerus

Duellman and Pramuk, 1999

Pristimantis angustilineata

Lynch, 1998

Pristimantis aniptopalmatus

Duellman and Hedges, 2005

Pristimantis anolirex

Lynch, 1983

Pristimantis anotis

Walker and Test, 1955

Pristimantis anthrax

Lynch, 2001

Pristimantis apiculatus

Lynch and Burrowes, 1990

Pristimantis appendiculatus

Werner, 1894

Pristimantis aracamuni

Barrio-Amores and Molina, 2006

Pristimantis ardalonychus

Duellman and Pramuk, 1999

Pristimantis ashkapara

Köhler, 2000

Pristimantis atrabracus

Duellman and Pramuk, 1999

Pristimantis atratus

Lynch, 1979

Pristimantis aurantiguttatus

Ruíz-Carranza, Lynch and Ardila-Robayo, 1997

Pristimantis aureolineatus

Guayasamin et al. 2006

Pristimantis avicuporum

Duellman and Pramuk, 1999

Pristimantis avius

Myers and Donnelly, 1997

Pristimantis bacchus

Lynch, 1984

Pristimantis baiotis

Lynch, 1998

Pristimantis balionotus

Lynch, 1979

Pristimantis baryecuus

Lynch, 1979

Pristimantis batrachites

Lynch, 2003

Pristimantis bearsei

Duellman, 1992

Pristimantis bellona

Lynch, 1992

Pristimantis bernali

Lynch, 1986

Pristimantis bicolor

Rueda-Almonacid and Lynch, 1983

Pristimantis bicumulus

Peters, 1863

Pristimantis bipunctatus

Duellman and Hedges, 2005

Pristimantis bisignatus

Werner, 1899

Pristimantis boconoensis

Rivero and Mayorga, 1973

Pristimantis bogotensis

Peters, 1863

Pristimantis boulengeri

Lynch, 1981

Pristimantis brevifrons

Lynch, 1981

Pristimantis briceni

Boulenger, 1903

Pristimantis bromeliaceus

Lynch, 1979

Pristimantis buccinator

Rodriguez, 1994

Pristimantis buckleyi

Boulenger, 1882

Pristimantis cabrerai

Cochran and Goin, 1970

Pristimantis cacao

Lynch, 1992

Pristimantis cajamarcensis

Barbour and Noble, 1920

Pristimantis calcaratus

Boulenger, 1908

Pristimantis calcarulatus

Lynch, 1976

Pristimantis caliginosus

Lynch, 1996

Pristimantis cantitans

Myers and Donnelly, 1996

Pristimantis capitonis

Lynch, 1998

Pristimantis caprifer

Lynch, 1977

Pristimantis carlossanchezi

Arroyo, 2007

Pristimantis carmelitae

Ruthven, 1922

Pristimantis carranguerorum

Lynch, 1994

Pristimantis carvalhoi

Lutz, 1952

Pristimantis caryophyllaceus

Barbour, 1928

Pristimantis cavernibardus

Myers and Donnelly, 1997

Pristimantis celator

Lynch, 1976

Pristimantis cerasinus

Cope, 1875

Pristimantis ceuthospilus

Duellman and Wild, 1993

Pristimantis chalceus

Peters, 1873

Pristimantis charlottevillensis

Kaiser, Dwyer, Feichtinger and Schmid, 1995

Pristimantis chiastonotus

Lynch and Hoogmoed, 1977

Pristimantis chloronotus

Lynch, 1969

Pristimantis chrysops

Lynch and Ruíz-Carranza, 1996

Pristimantis citriogaster

Duellman, 1992

Pristimantis colodactylus

Lynch, 1979

Pristimantis colomai

Lynch and Duellman, 1997

Pristimantis colostichos

La Marca and Smith, 1982

Pristimantis condor

Lynch and Duellman, 1980

Pristimantis conspicillatus

Günther, 1858

Pristimantis cordovae

Lehr and Duellman, 2007

Pristimantis corniger

Lynch and Suárez-Mayorga, 2003

Pristimantis corrugatus

Duellman, Lehr, and Venegas, 2006

Pristimantis cosnipatae

Duellman, 1978

Pristimantis cremnobates

Lynch and Duellman, 1980

Pristimantis crenunguis

Lynch, 1976

Pristimantis crepitans

Bokermann, 1965

Pristimantis cristinae

Lynch and Ruíz-Carranza, 1985

Pristimantis croceoinguinis

Lynch, 1968

Pristimantis crucifer

Boulenger, 1899

Pristimantis cruciocularis

Lehr, Lundberg, Aguilar, and von May, 2006

Pristimantis cruentus

Peters, 1873

Pristimantis cryophilius

Lynch, 1979

Pristimantis cryptomelas

Lynch, 1979

Pristimantis cuentasi

Lynch, 2003

Pristimantis cuneirostris

Duellman and Pramuk, 1999

Pristimantis curtipes

Boulenger, 1882

Pristimantis danae

Duellman, 1978

Pristimantis degener

Lynch and Duellman, 1997

Pristimantis deinops

Lynch, 1996

Pristimantis delicatus

Ruthven, 1917

Pristimantis delius

Duellman and Mendelson, 1995

Pristimantis devillei

Boulenger, 1880

Pristimantis diadematus

Jiménez de la Espada, 1875

Pristimantis diaphonus

Lynch, 1986

Pristimantis diastema

Cope, 1875

Pristimantis diogenes

Lynch and Ruíz-Carranza, 1996

Pristimantis dissimulatus

Lynch and Duellman, 1997

Pristimantis dorsopictus

Rivero and Serna, 1988

Pristimantis douglasi

Lynch, 1996

Pristimantis duellmani

Lynch, 1980

Pristimantis duende

Lynch, 2001

Pristimantis dundeei

Heyer and Muñoz, 1999

Pristimantis elegans

Peters, 1863

Pristimantis epacrus

Lynch and Suárez-Mayorga, 2000

Pristimantis eremitus

Lynch, 1980

Pristimantis eriphus

Lynch and Duellman, 1980

Pristimantis ernesti

Flores, 1987

Pristimantis erythropleura

Boulenger, 1896

Pristimantis esmeraldas

Guayasamin, 2004

Pristimantis eugeniae

Lynch and Duellman, 1997

Pristimantis euphronides

Schwartz, 1967

Pristimantis eurydactylus

Hedges and Schlüter, 1992

Pristimantis exoristus

Duellman and Pramuk, 1999

Pristimantis factiosus

Lynch and Rueda-Almonacid, 1998

Pristimantis fallax

Lynch and Rueda-Almonacid, 1999

Pristimantis fenestratus

Steindachner, 1864

Pristimantis festae

Peracca, 1904

Pristimantis fetosus

Lynch and Rueda-Almonacid, 1998

Pristimantis flavobracatus

Lehr, Lundberg, Aguilar, and von May, 2006

Pristimantis floridus

Lynch and Duellman, 1997

Pristimantis frater

Werner, 1899

Pristimantis fraudator

Lynch and McDiarmid, 1987

Pristimantis gaigeae

Dunn, 1931

Pristimantis galdi

Jiménez de la Espada, 1871

Pristimantis ganonotus

Duellman and Lynch, 1988

Pristimantis gentryi

Lynch and Duellman, 1997

Pristimantis ginesi

Rivero, 1964

Pristimantis gladiator

Lynch, 1976

Pristimantis glandulosus

Boulenger, 1880

Pristimantis gracilis

Lynch, 1986

Pristimantis grandiceps

Lynch, 1984

Pristimantis gularis

Boulenger, 1898

Pristimantis gutturalis

Hoogmoed, Lynch and Lescure, 1977

Pristimantis hamiotae

Flores, 1994

Pristimantis hectus

Lynch and Burrowes, 1990

Pristimantis helvolus

Lynch and Rueda-Almonacid, 1998

Pristimantis hernandezi

Lynch and Ruíz-Carranza, 1983

Pristimantis huicundo

Guayasamin, Almeida-Reinoso, and Nogales-Sornosa, 2004

Pristimantis hybotragus

Lynch, 1992

Pristimantis hylaeformis

Cope, 1875

Pristimantis ignicolor

Lynch and Duellman, 1980

Pristimantis illotus

Lynch and Duellman, 1997

Pristimantis imitatrix

Duellman, 1978

Pristimantis incanus

Lynch and Duellman, 1980

Pristimantis incertus

Lutz, 1927

Pristimantis incomptus

Lynch and Duellman, 1980

Pristimantis infraguttatus

Duellman and Pramuk, 1999

Pristimantis inguinalis

Parker, 1940

Pristimantis insignitus

Ruthven, 1917

Pristimantis inusitatus

Lynch and Duellman, 1980

Pristimantis ixalus

Lynch, 2003

Pristimantis jaimei

Lynch, 1992

Pristimantis johannesdei

Rivero and Serna, 1988

Pristimantis jorgevelosai

Lynch, 1994

Pristimantis juanchoi

Lynch, 1996

Pristimantis jubatus

Garcia and Lynch, 2006

Pristimantis kareliae

La Marca, 2005

Pristimantis katoptroides